Table 1 Primer sequences and their products
rs16260 | rs157180 | |
|---|---|---|
Forward inner | CCAAGCAACTCCAGGCTAGAGGGTTAC | ACAGAAAGGTAAAGAAAGTTTCAAAGTG |
Reverse inner | CCACCCAGCCTCGCATAGACGTGT | GTGAGTACATACTGGCTTTTCCATACA |
Forward outer | TGTCCGCCCCGACTTGTCTCTCTAC | AGTCTGAGTCAACTGTTCTAGAGGCTAA |
Reverse outer | GGTGCTTTGCAGTTCCGACGCCACT | TTCTAATTATAGCACTCCAGAGGGAAC |
Primer products | A (mutant) = 397 bp C (wild) = 212 bp A/C = 397/212 bp C + = 559 bp | G (mutant) = 449 bp T (wild) = 181 bp G/T = 449/181 bp C + = 576 bp |