The role of miRNAs in the diagnosis and treatment of male infertility: a review study

Egyptian Journal of Medical Human Genetics

Table 2 MicroRNAs with biomarker capability for male infertility in previous studies

MirRNAs	Type of patients	Analysis method	Up/down-regulating	Sample type for RNA isolation	Refs.
has-miR-9-3p has-miR-30b-5p has-miR-122-5p	Infertile men	RT-PCR	−	Sperm	[32]
miR-26a-5p	Infertile men, normozoospermia	RT-qPCR	Down	Ejaculated sperm	[33]
miR-34/449 family	Infertile men	–	–	–	[37]
has-miR-34b^* has-miR-34b has-miR-34c-5p has-miR-429 has-miR-122	Subfertile, nonobstructive azoospermia	RT-qPCR	Down Down Down Up Down	Semen	[34]
miR-20a-5p	Nonobstructive azoospermia, normozoospermia	RT-qPCR	Up	Blood plasma	[38]
miR-182-5p miR-192-5p miR493-5p	Teratozoospermia infertile men	RT-PCR	Up Up Up	Semen	[35]
miR-34c-5p	Infertile men	RT-qPCR	up	Seminal plasma	[36]
miR-383 miR-122 miR-15b	Infertile men: SOAT, MOAT, NOA, OA	Diff-Quick, RT-qPCR	Up Up Up	Sperm and testicular tissue	[39]
has-miR-942-5p/has-miR-1208 has-miR34b-3p/has-miR93-3p	Asthenozoospermia Teratozoospermia, Oligozoospermia, unexplained male infertility [UMI]	RT-qPCR	–	Semen	[40]
miR-210-3p	varicocele	RT-PCR	Up	Semen	[41]
miR-141 miR-429 miR-7–1-3p	Nonobstructive azoospermia	Tagman RT-qPCR assay	Up Up UP	Seminal plasma	[42]
miR-26b miR-374b	Normospermia	NGS	Down Down	Seminal plasma	[43]
miR-371a-3p	Oligozoospermia	qPCR	Down	Unprocessed ejaculate	[44]
miR-192a	Nonobstructive azoospermia and varicoceles	RT-qPCR	Down	Seminal plasma an d testicular tissue
miR-155	Hypogonadal and eugonadal	RT-PCR	–	Cell-free serum
miR-27a	Asthenotertozoo-spermia, normozoospermia	RT-qPCR Western blot	Up	Semen