Table 1 PCR primers
From: The role of miRNA-29b1, MMP-2, MMP-9 mRNAs, and proteins in early diagnosis of HCC
Gene | Sequence | Gene bank accession number |
|---|---|---|
MMP-2 | Forward primer. 5−CTCATCGCAGATGCCTGGAA-3− Reverse primer. 5−-TTCAGGTAATAGGCACCCTTGAAGA-3− | NM_001302510.1 |
MMP-9 | Forward primer. 5′-ACGCACGACGTCTTCCAGTA-3′ Reverse primer. 5′-CCACCTGGTTCAACTCACTCC-3′ | NG_011468.1 |
miRNA-29b1 | Forward primer. 5′--GCTGGTTTCATATGGTGG-3′ Reverse primer. 5′-GAACATGTCTGCGTATCTC-3′ | NR_029517.1 |
GAPDH | Forward primer 5′-CCTGAGGGTTCTTTGTGCTGA-3′ Reverse primer 5′-AAAGGCTCAACCTTCCCCAT-3′ | NG_007073.2 |
RNU6 snRNA | Forward 5′-CTCGCTTCGGCAGCACAT-3′ Reverse 5′-TTTGCGTGTCATCCTTGCG-3′ | NC_000001.11 |