Table 1 The primer pairs for each SNP and their characteristics
Gene | SNP | Primer pairs | PCR conditions | Product size ( bp) |
|---|---|---|---|---|
EGFR | rs712829 | F: 5′-GAGCTAGACGTCCGGGCA-3′ 5′-GCTCTCCCGATCAATACTGGA-3′ R: | 35 cycles: 94 °C for 10 min, 94 °C for 1 min, 64 °C for 45 s, 72 °C for 45 s, 72 °C for 5 min | 240 |
Exo1 | rs1047840 | 5′-GACACAGATGTAGCACGTAA-3′ F: 5′-CTGCGACACATCAGACATAT-3′ R: | 35 cycles: 94 °C for 10 min, 94 °C for 1 min, 56 °C for 45 s, 72 °C for 45 s and 72 °C for 5 min | 306 |
LEP | rs7799039 | 5′-TAAGCCAAGGCAAAATTGAG-3′ F: 5′-CTTCAAAATTTATGTTCCTCTGC-3′ R: | 35 cycles: 94 °C for 10 min, 94 °C for 1 min, 55 °C for 45 s,72 °C for 45 s and 72 °C for 5 min | 281 |