Table 5 A summary of PCR methods and their associated conditions
From: Association of ADAM33 gene with COPD pathophysiology: a case–control study
Step | Temperature °C | Time | Number of cycles |
|---|---|---|---|
Initial Denaturation | 94 | 5 min | 1 |
Denaturation | 94 | 30 s | 40 |
Annealing | 60.45 | 30 s | |
Extension | 72 | 1 min/kb | |
Final Extension | 72 | 10 min | 1 |