Table 1 Sequence of primers used in this research
From: Effect of Dioscorea extract on Bax and Bcl-2 gene expression in MCF-7 and HFF cell lines
Gene | Primer pair sequences | Tm (°C) | GC% | Accession N |
|---|---|---|---|---|
β-actin (F) | 5′-TCCTCCTGAGCGCAAGTAC-3′ | 59.11 | 57.89 | NM-011001.5 |
β-actin (R) | 5′-CCTGCTTGCTGATCCACATCT-3′ | 60.41 | 52.38 | NM-011001.5 |
Bax (F) | 5′-GAGCTGCAGAGGATGATTGC-3′ | 59.05 | 55.00 | NM-138764.5 |
Bax (R) | 5′-AAGTTGCCGTCAGAAAACATG-3′ | 57.65 | 42.86 | NM-138764.5 |
Bcl-2 (F) | 5′-ATTGGGAAGTTTCAAATCAGC-3′ | 54.98 | 38.10 | NM-000657.3 |
Bcl-2 (R) | 5′-CAGTCTACTTCCTCTGTGATGTTG-3′ | 58.83 | 45.83 | NM-000657.3 |