Table 2 PCR steps and repeat cycle
From: Effect of Dioscorea extract on Bax and Bcl-2 gene expression in MCF-7 and HFF cell lines
Stages | Temperature (°C) | Time (s) | Number of cycles |
|---|---|---|---|
Denaturation | 95 | 10 | 40 |
Annealing | 55 | 20 | |
Extension | 72 | 20 |
From: Effect of Dioscorea extract on Bax and Bcl-2 gene expression in MCF-7 and HFF cell lines
Stages | Temperature (°C) | Time (s) | Number of cycles |
|---|---|---|---|
Denaturation | 95 | 10 | 40 |
Annealing | 55 | 20 | |
Extension | 72 | 20 |