Table 1 Primers of miRNA-20a and miRNA 320 used in quantitative RT– PCR
From: The role of miRNA20a and miRNA320 in Iraqi patients with COVID-19: a case–control study
Primers of miRNA-20a and miRNA-U6 | Primer sequence (5ʹ → 3ʹ direction) |
|---|---|
miRNA-20a primers | Forward: GCCCGCTAAAGTGCTTATAGTG |
Reverse: CCAGTGCAGGGTCCGAGGT | |
miRNA-20a RT primer | GTCGTATCCAGTGCAGGGTCCGAGGTGCACTGGATACGACCTACCTG |
miRNA-U6 RT primer | GTCGTATCCAGTGCAGGGTCCGAGGTGCACTGGATACGACAAAATATGG |
miRNA-U6 Forward primer | TGCGGGTGCTCGCTTCGGCAGC |
Primers of miRNA-320 and miRNA-U6 | Primer sequence (5ʹ → 3ʹ direction) |
miRNA-320 primers | Forward: AAAAGCTGGGTTGAGAGGGCGA |
Reverse: CAGTGCGTGTCGTGGAGT | |
miRNA-320 RT primer | GCTTCGCTCCCCTCCGCCTTCTCTTCCCGGTTCTTCCC |
GGAGTCGGGAAAAGCTGGGTTGAGAGGGCGAAAAAGGATGAGGT | |
miRNA-U6 RT primer | GTCGTATCCAGTGCAGGGTCCGAGGTGCACTGGATACGACAAAATATGG |
miRNA-U6 Forward primer | TGCGGGTGCTCGCTTCGGCAGC |