Table 1 Sequences of the primers used in real-time PCR assay
Genes | Sequence primers | Tm |
|---|---|---|
Col4A5 | F: ACTATGCCAACTCCTACAGC | 55 |
R: TTCATGCACACTTGACATCGG | ||
SPI | F: CTCCTTCAGCCCTTATTACCAC | 58 |
R:ATTGCCACTGATATTAATGGACTGC | ||
IGF2BP3 | F:ATTCACAGAAGAGATCCCCTTGA | 58 |
R: ATTCCTGCAATGGAGATATCGTGA | ||
LIN28A | F: AAGGGTCTGGAATCCATCCGTGT | 61 |
R: AGACCTCCACAGTTGTAGCACC | ||
BCL2 | F: CGACGACTTCTCCCGCCGCTA | 62 |
R: GACATCTCCCGGTTGACGCTCT |