Egyptian Journal of Medical Human Genetics

Table 2 Programs used to measure the expression of different genes by real-time PCR

From: Effect of hydroxyurea on SP1, LIN28B, IGF2BP3, COL4A5, BCL2, gamma globin genes expression: an in vitro study

Gene	Step 1	Step 2 (30 cycles)	Step 3	Step 4
SP1	95 °C; 5 min	95 °C–60 s	72 °C; 5 min	60 °C; 1 min
		58 °C–45 s
		72 °C–45 s
IGF2BP3		95 °C–60 s
		58 °C–45 s
		72 °C–45 s
LIN28A		95 °C–60 s
		61 °C–30 s
		72 °C–30 s
COL4A5		95 °C–60 s
		55 °C–20 s
		72 °C–30 s
BCL2		95 °C–60 s
		62 °C–30 s
		72 °C–30 s

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