Table 1 Primers sequences, expected PCR product sizes, annealing conditions and restriction enzymes used
Gene | SNPs | Forward (F) and reverse (R) primers sequences | PCR products size (bp) | Annealing steps for PCR | Restriction enzyme (incubation condition) |
|---|---|---|---|---|---|
ABCG2 | rs2231142 | F = 5′-GTCTCATTAAAATGCTATTT-3′ | 151 | 49.2 °C for 35 s | MseI, (37 °C for 1 h) |
R = 5′-CTCTTGAATGACCCTGTTGA-3′ | |||||
ABCC2 | rs717620 | F = 5′-TGTCCATCCACTGTTTCAATG-3′ | 193 | 54.2 °C for 1 min | TaqαI (65 °C for 1 h) |
R = 5′-CTGGACTGCGTCTGGAT*C-3′ | |||||
APOE [56] | rs429358 | F = 5′-TCCAAGGAGCTGCAGGCGGCGCA-3′ | 218 | 60 °C for 90 s | HaeII, AflIII (37 °C for 1 h) |
rs7412 | R = 5′-GCCCCGGCCTGGTACACTGCCA-3′ | ||||
GATM | rs9806699 | F = 5′-CAAGCTGCCAACTTCCATCT-3′ | 225 | 56.9 °C for 1 min | SduI (37 °C for 1 h) |
R = 5′-CCCTCAGAATGGTGACATCC-3′ | |||||
COQ2 | rs4693075 | F = 5′-CCACAACTTTCCCACAAATC-3′ | 219 | 54.0 °C for 30 s | CviKI-1 (37 °C for 1 h) |
R = 5′-TGGTGCGGTGTAGGTATTGA-3′ | |||||
APOA5 [57] | rs662799 | F = 5′-GATTGATTCAAGATGCATTTAGGAC-3′ | 187 | 55 °C for 30 s | MseI (37 °C for 1 h) |
R = 5′-CCCCAGGAACTGGAGCGAAATT-3′ |