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Vici syndrome in an Egyptian infant: case report and differential diagnosis of inherited hypopigmented disorders



Vici syndrome is a severe inherited multisystem disease caused by mutations in the EPG5 gene. The diagnosis depends on the constellation of cardinal features of agenesis of the corpus callosum, cataracts, oculocutaneous hypopigmentation, cardiomyopathy, and a combined immunodeficiency followed by confirmation by genetic testing. We report an Egyptian infant with Vici syndrome carrying a homozygous splice site variant (c.1252+1G>T; NM_020964.2) in the EPG5 gene, detailed clinical description, outcome, and differential diagnosis of inherited hypopigmentation disorders associated with neurological manifestations.

Case presentation

The infant initially presented with oculocutaneous hypopigmentation, agenesis of the corpus callosum, and immunodeficiency. A few months later, a diagnosis of dilated cardiomyopathy was made. Family history revealed 2 deceased siblings phenotypically matching our index infant. He died at the age of 15 months with acute respiratory failure.


The accurate diagnosis of such rare diseases with genetic confirmation is vital for proper clinical decision-making, genetic counseling of the affected families, and future genotype-phenotype correlation studies.


Vici syndrome (OMIM 242840) is a severe autosomal recessive multisystem disease characterized by agenesis of the corpus callosum, cataracts, oculocutaneous hypopigmentation, cardiomyopathy, and combined immunodeficiency. This rare disorder was first described by Dionisi Vici and colleagues in 1988 [1], and since then, 82 genetically confirmed cases have been reported, not including ours [2,3,4].

Mutations of the ectopic P-granules autophagy protein 5 homolog (EPG5) gene, on chromosome 18q, were first documented as the underlying etiology of Vici syndrome in 2013 [5]. The EPG5 protein has a key role as a regulator of autophagy, which is a fundamental cellular degradative pathway. This process is enhanced in the neurons and muscles, which might explain the prominent CNS, neuromuscular involvement, and multisystem affection in patients with Vici syndrome [6]. To date, nearly 40 different EPG5 mutations have been identified without clear genotype-phenotype correlations [6].

We report an Egyptian infant with genetically confirmed Vici syndrome with a detailed clinical description, course of the disease, genetic mutations, and differential diagnosis of some of the inherited hypopigmentation disorders associated with neurological manifestations (Table 1).

Table 1 Differential diagnosis inherited hypopigmentation disorders associated with neurological manifestations

Case presentation

At the time of examination, our index patient was a 2-month-old male infant born to consanguineous parents (first-degree cousins). Family history revealed 2 deceased siblings; a male and a female infant who had passed away at the age of 3 and 2 months, respectively. The male sibling died after a few days of acute gastroenteritis with severe dehydration complicated by septic shock. The female sibling died due to severe acute bacterial meningitis. Both deceased siblings had characteristic hypopigmented hair and skin, phenotypically matching our index infant but not the parents, the healthy 10-year-old brother, or other family members (Fig. 1).

Fig. 1
figure 1

a The phenotype of the index case shows hypopigmented hair and skin, b tuft of hair on the index patient’s lower back over the sacral region, c the family pedigree, and d hair trichogram showing an absent medulla and a decrease in pigmentation.

The antenatal history was uneventful; he was born at term by normal vaginal delivery. However, postnatally, he was admitted to the neonatal intensive care unit (NICU) for 10 days immediately after birth due to early-onset sepsis as blood culture revealed Klebsiella pneumoniae (K. pneumoniae). Following discharge from the NICU, he spent only 2 days at home and was readmitted for 20 days with fever and refusal of feeding. His initial examination showed characteristic fair hair and skin with dysmorphic facial features including receding mandible and low-set ears. His anthropometric measures for weight and height were decreased for his age and sex: body weight = 3 kg (Z score = − 3.97), length = 55 cm (Z score = − 2), while head circumference = 38 cm (Z score = − 1.63). Generally, he was hypoactive and visually inattentive with generalized hypotonia, a tuft of hair on his lower back over the sacral region, and talipes equinovarus of the left foot (Fig. 1). A trans-cranial ultrasound revealed agenesis of the corpus callosum, and his echocardiogram showed patent foramen oval (PFO). Vici syndrome was suspected, and further investigations were requested.

Laboratory investigation including complete blood count (CBC), renal and liver function tests, serum lactate, pyruvate, ammonia, extended metabolic screen, and urinary organic acid profile were all within the normal range. His immunological profile including T and B cell quantitative analysis confirmed that the total number of T cells and the CD8+ cell population was decreased compared to the normal ranges corresponding to the patient’s age. On the other hand, his immunoglobulin profile was normal for his age: IgG 452 mg/dl (normal 445–1050), IgA 37 mg/dl (normal 20–60), and IgM 63 mg/dl (normal 30–80). Workup also included a hair trichogram, which showed an absent medulla and a decrease in pigmentation (Fig. 1).

Brain magnetic resonance imaging (MRI) showed evidence of agenesis of the corpus callosum (ACC) and atrophic brain changes, while his spine MRI revealed normal cord and spine structure with no evidence of neural tube defects. The ophthalmological evaluation reported hypopigmented fundus and decreased foveal reflex. An Auditory Brainstem Response test (ABR) showed moderate to severe hearing loss at high frequencies (2–4 kHz) in his left ear.

Following the parents’ written consent, blood samples of the patient and his parents were drawn, and DNA was extracted. Targeted next-generation sequencing of EPG5 revealed a homozygous c.1252+1G>T (NM_020964.2) variant in our patient. This change was classified as a likely pathogenic change [7], was found in the Genome Aggregation Database (gnomAD) only once and in a heterozygous state (allele frequency 0.000004013), and has a Combined Annotation Dependent Depletion (CADD) score of 33 [8]. Using targeted Sanger sequencing, the same alteration was detected in each of the parents in a heterozygous state.

By the age of 6 months, he was admitted due to respiratory distress, and a diagnosis of dilated cardiomyopathy was made as his echocardiography (ECHO) revealed significant left ventricular end-diastolic dilation with decreased ejection fraction (EF = 40%) for which Lanoxin (digoxin) and diuretics (Frusemide) were prescribed. Due to frequent infections, the patient was put on a prophylactic dose of sulfamethoxazole and trimethoprim (150 mg/m2/day) every other day and monthly intravenous immunoglobulin (IVIG) (400 mg/kg once every 4 weeks). By the age of 12 months, he experienced repeated hospital admissions due to recurrent respiratory and gastrointestinal tract infections. Acute infections were treated with broad-spectrum antibiotics (including ceftriaxone, meropenem, vancomycin, fluconazole) and intravenous immunoglobulin IVIG (400 mg/kg/day for 5 days). Despite the abovementioned treatments, he died at the age of 15 months from acute respiratory failure due to extensive acute bacterial pneumonia.


Vici syndrome is a rare hereditary disease with a diagnosis that depends on the constellation of five main cardinal features: agenesis of the corpus callosum, cataracts, oculocutaneous hypopigmentation, cardiomyopathy, and a combined immunodeficiency, which are found in almost all reported cases of Vici syndrome [6]. In addition, more recently recognized but equally consistent features include profound developmental delay, progressive microcephaly, and failure to thrive [9]. Furthermore, a wide range of additional findings has been reported in isolated cases including features such as hearing loss, lung hypoplasia, renal tubular necrosis, idiopathic thrombocytopenic purpura, and myopathy [10,11,12,13].

The initial presentation of our case included four out of the five classical features: oculocutaneous hypopigmentation, immunodeficiency, and agenesis of the corpus callosum. Cardiomyopathy developed a few months later following his initial presentation and is consistent with other reported cases in which initial echocardiography was normal. Thus, regular follow-up with ECHO for any suspected case of Vici syndrome is of crucial importance [14].

Moreover, our patient had profound developmental delay and failure to thrive, representing six out of the eight key features that were found to have a specificity of 97% and a sensitivity of 89% for cases with a positive EPG5 genetic test [9]. Other less common manifestations in our case included facial dysmorphism in the form of receding mandible and low-set ears, reported by Said [15]. and Vojcek et al. [4]. Other dysmorphic features reported in some children with Vici syndrome include cleft lip and palate, hypertelorism, high-arched palate, micrognathia [1, 13, 16, 17], coarse facial features [5, 9], small anterior fontanelle with overlapping sutures, and broad nose [10], and Epicanthal folds were being reported less frequently [3].

Our index case had a hearing defect, which has been reported in a very few cases, specifically the sensory neural hearing loss subtype (SNHL) [4, 10, 17, 18]. However, we believe that it might be frequently unrecognized because of the overwhelming multisystem effects of Vici syndrome that may present very early in life and cause early death. Therefore, although ABR was not included as a baseline investigation for the diagnosis of a patient with suspected Vici syndrome, it should be investigated [6]. Moreover, the index case showed a tuft of hair on his lower back over the sacral region and talipes equinovarus of the left foot, which are reported here for the first time in Vici syndrome.

Other neurological manifestations of Vici syndrome, besides developmental delay, hearing deficits, and hypotonia documented in our case, include seizures, myopathy, progressive microcephaly, and neuropathy which have been reported in more than half of the children [6]. CNS affection is not fully explained by the associated structural brain lesion such as ACC, pontine hypoplasia, and others, as recent studies have suggested that Vici syndrome has a neurodegenerative pattern with progressive loss of skills and profound acquired microcephaly. Furthermore, the EPG5 genes were linked to early-onset epileptic encephalopathies, respiratory chain enzyme abnormalities, and secondary mitochondrial dysfunction as a possible downstream effect of defective autophagy [9].

The genetic testing of our patient revealed a homozygous likely pathogenic variant in the EPG5 gene confirming the clinical diagnosis of Vici syndrome. The c.1252+1G>T substitution that we discovered affects the first nucleotide of intron 3 and, to date, has not been reported in Vici syndrome patients. It is predicted to disrupt the highly conserved donor splice site [19, 20] and cause skipping of exon 3 and the complete loss of functional EPG5 protein either through protein truncation or, more likely, through nonsense-mediated decay. In line with this finding, the majority of the previously reported EPG5 pathogenic variants are nonsense, frameshift, and splice site changes indicating a loss of function mechanism in Vici syndrome patients. Unfortunately, no RNA or cell sample was available from the parents of the index patient or any other family member potentially carrying the c.1252+1G>T change. Thus, we were unable to experimentally validate the occurrence of altered splicing and nonsense-mediated RNA decay.

As for clinicians, suspicion of Vici syndrome is based on the characteristic clinical manifestations. Differential diagnosis based on other syndromes showing phenotypical overlap with Vici syndrome [6], or other neurometabolic etiologies of agenesis of the corpus callosum [21], has been suggested. One approach is to be aware of other differential diagnoses particularly other inherited hypopigmentation disorders, and especially those associated with neurological manifestations and structural brain lesions. This approach is a real challenge due to overlapping features. These rare disorders could include gray hair syndromes like Griscelli syndrome (GS), Chediak-Higashi syndrome (CHS), and oculocerebral hypopigmentation syndrome, cross-type (OHS). However, they all share the presence of silvery gray hair and the characteristic hair trichogram which is not commonly found in cases of Vici syndrome. Besides, CHS is characterized by giant azurophilic granules within neutrophils and regular small melanin granules visible in a trichogram while the neurological manifestations are either primary (developmental and degenerative) or secondary to an accelerated phase of hemophagocytic lymphohistiocytosis (CNS-HLH) [22]. Unlike CHS, the trichogram of GS shows small and large unevenly distributed melanin clumps. Neurological manifestations are found in all types except for GS type 3 [23]. Neurological presentations of GS1 are mostly primary due to myosin-Va deficiency [24], while those of GS2 are either due to CNS infections, due to a consequence of the associated immune deficiency, or due to CNS-HLH [25]. Although hypopigmentation may not be a consistent major finding in both Prader-Willi and Angelman syndromes, it should be included in the differential diagnosis and can be recognized by their characteristic somatic and behavioral problems [26, 27]. Laboratory investigations might narrow the list such as in cases of suspected phenylketonuria, which can be excluded by phenylalanine level in the blood and urine, and Menkes syndrome by measuring serum copper and ceruloplasmin in a child with short friable sparse hair (Table 1).

Counseling for the parents involved discussing their concerns about themselves, their healthy older child, and their future pregnancy probabilities. They were told that they were both carriers which means that both only have a genetic change that does not cause any health problems for them. For their older healthy child, they were advised to perform molecular testing to determine if he was a carrier or not affected. But the most important concerns for them were about the next pregnancies; genetic counseling was provided, and they were advised to perform prenatal diagnosis if they are intending to have other children.

Regarding patient prognosis, Vici syndrome is a devastating neurodegenerative disease with progressive multisystem pathology. The prognosis is variable with reported attenuated Vici syndrome phenotype [28]. However, most of the cases had a poor prognosis and a median survival time of 24 months. Cardiomyopathy and recurrent infections, the causes of death of the current case, are the most common causes of death [9].


The accurate diagnosis of such a rare disease with molecular confirmation is vital for proper clinical decision-making, genetic counseling of the affected families, and future genotype-phenotype correlation studies.

Availability of data and materials

All data used during this report are included in this published article. Further data are available from the corresponding author on reasonable request.



Auditory Brainstem Response test


Agenesis of the corpus callosum


Autosomal recessive


Combined Annotation-Dependent Depletion


Complete blood count


Cluster of differentiation


Chediak-Higashi syndrome


Central nervous system


Deoxyribonucleic acid




Ejection fraction


Ectopic P-granules autophagy protein 5


Gray hair syndromes


Genome Aggregation Database


Griscelli syndrome


Hemophagocytic lymphohistiocytosis


Immunoglobulin A


Immunoglobulin G


Immunoglobulin M


Intravenous immunoglobulin


Left ventricular end-diastolic dilatation


Magnetic resonance imaging




Neonatal intensive care unit


Oculocerebral hypopigmentation syndrome


Phenylalanine hydroxylase


Patent foramen oval


Sensory neural hearing loss


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The authors would like to thank the parents of the presented case for their approval of participation and the kind contribution of their samples.



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MAM clinically followed up the index case regarding his neurological manifestations. AA clinically diagnosed and followed up the index case. CP conducted the genetic analysis of the index case and his parents. MD conducted the hair trichogram. All authors participated in the writing and revision process of the manuscript. All authors have read and approved the final manuscript.

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CP is an employee of CENTOGENE AG (Rostock, Germany).

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Correspondence to Marwa Abd Elmaksoud.

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The manuscript had the approval of the Ethical Committee of the Faculty of Medicine, Alexandria University. Written informed consent was taken from the parent of the index case (legal guardian) for participation.

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Written consent was taken from the parent of the index case for publication.

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The authors declare no competing interest.

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Abd Elmaksoud, M., Abeesh, A.A., Pereira, C. et al. Vici syndrome in an Egyptian infant: case report and differential diagnosis of inherited hypopigmented disorders. Egypt J Med Hum Genet 21, 59 (2020).

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