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Table 4 Calculated physiochemical properties by the ExPASy ProtParam server

From: Characterization of the SARS-CoV-2 coronavirus X4-like accessory protein

Calculated parameters

Template protein

Model protein

Molecular weight

9896.10

9478.71

Theoretical pI

7.06

6.32

Amino acid composition (total)

87

83

Atomic composition

C438H667N123O132S4

C426H644N112O126S4

Extinction coefficient

7700

7700

Estimated half-life

30 h (mammalian reticulocytes, in vitro).

> 20 h (yeast, in vivo).

> 10 h (Escherichia coli, in vivo).

30 h (mammalian reticulocytes, in vitro).

> 20 h (yeast, in vivo).

> 10 h (Escherichia coli, in vivo).

Instability index

66.61

56.58

Aliphatic index

60.57

69.28

GRAVY

− 0.569

− 0.343