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Egyptian Journal of Medical Human Genetics

Table 1 Primers used in T‐ARMS‐PCR of glutathione S-transferase P1 gene (A/G rs1695)

From: Impact of gene polymorphism of glutathione S-transferase and ghrelin as a risk factor in Egyptian women with gestational diabetes mellitus

Primer

Sequence (5′–3′)

Fragment size

FOP

CAGGTGTCA GGTGAGCTCTGAGCA CC

A allele 233 bp (FIP + ROP)

ROP

ATAAGGGTG CAGGTTGTGTCTTGTCCC

G allele 290 bp (FOP + RIP)

FIP

CGTGGAGGACCTCCGCTGCAAATCCA

Two outer primers 467

RIP

GCTCACATAGTTGGTGTAGATGAGGGATAC

 
  1. FOR forward outer primer, ROP reverse outer primer, FIR forward inner primer, RIP reverse inner primer
  2. aThe mismatches of the allele-specific primers are emphasized in bold and underlined
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