Mutational analysis of phospholipase C epsilon 1 gene in Egyptian children with steroid-resistant nephrotic syndrome

Egyptian Journal of Medical Human Genetics

Table 10 Structure-Based stability Predictions

Mutation	PremPS (ΔΔG)	DYNAMUT	ΔΔG mCSM	ΔΔG SDM	ΔΔG DUET	ΔΔS_Vib ENCoM
R1230H	1.68 kcal/mol (Stabilizing) Core location	− 0.529 kcal/mol (Destabilizing)	− 1.450 kcal/mol (Destabilizing)	0.890 kcal/mol (Stabilizing)	− 1.178 kcal/mol (Destabilizing)	0.222 kcal.mol-1.K-1 (Increase of molecule flexibility)
E1393K	0.4 kcal/mol (Stabilizing) Surface location	0.156 kcal/mol (Stabilizing)	0.171 kcal/mol (Stabilizing)	− 1.000 kcal/mol (Destabilizing)	0.387 kcal/mol (Stabilizing)	− 0.043 kcal.mol-1.K1 (Decrease of molecule flexibility)