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Association study of APOE gene polymorphisms with diabetes and the main cardiometabolic risk factors, in the Algerian population
Egyptian Journal of Medical Human Genetics volume 20, Article number: 5 (2019)
Metabolic syndrome (MetS) represents a combination of at least three primary metabolic abnormalities among which obesity, hyperglycemia, dyslipidemia, and high blood pressure (HBP); once combined, they increase the cardiovascular risk significantly. APOE gene is considered as a genetic risk factor for cardiovascular diseases, and it has been linked to MetS or related disorders in several populations. Our study aimed to analyze, for the first time, the association of three APOE gene polymorphisms with MetS risk and its components in a general Algerian population sample, and to highlight the potential influence of these polymorphisms on individual susceptibility to MetS, diabetes, high blood pressure, and obesity, which has never been studied before in the Algerian population.
The rs439401 showed a significant association with hypertension. The T allele confers a high risk of hypertension with an odds ratio (OR) of 1.46 (95% CI [1.12–1.9], p = 0.006). The rs4420638 polymorphism was significantly associated with obesity in the general population. The G allele provides protection against obesity; the resulting OR is 0.48 (95% CI [0.29–0.81], p = 0.004).
Although APOE variants were not associated with the risk of MetS, the APOE polymorphism alleles were associated with some of the metabolic parameters in Algerian subjects. The relation of APOE rs439401 alleles with high blood pressure seems indicative of a state of stress of the population.
The concept of the metabolic syndrome (MetS) emerged following the increase of the risk factors associated with cardiovascular diseases and diabetes [1, 2]. MetS represents a combination of at least three primary metabolic abnormalities among which obesity, hyperglycemia, dyslipidemia, and high blood pressure (HBP); once combined, they significantly increase the risk of cardiovascular diseases [3,4,5,6,7].
In Algeria, the health network improvement led to a progressive aging of the population which allows for the emergence of abnormalities associated with aging and MetS. The TAHINA study (Epidemiological Transition And Health Impact in North Africa) conducted in 2005 showed a high prevalence of hypertension (24.9%) and diabetes (12.2%) in the Algerian population. Overweight has become a real public health problem, especially among women, with 66.5% overweight and 30.1% obese women. Cardiovascular disease and diabetes accounted for 26.1% and 4.4% of deaths, respectively, in 2002 .
There have been at least six different published definitions for MetS; the most common definition is that of the National Cholesterol Education Program Adult Treatment Panel III (NCEP ATP III) . The prevalence of MetS differs according to several parameters: definition, country, sex, age, and even according to the region in the same country. In Algeria, a recent study shows that the prevalence of metabolic syndrome according to the National Cholesterol Education Program - Adult Treatment Panel (NCEP-ATP) III definition was 20% in the Oran population; it was higher in women than in men (25.9 vs 13.7%) .
Metabolic syndrome is a multifactorial disease that implicates both environmental and genetic factors . Given the importance of APOE in the metabolism of lipoproteins, indeed, APOE gene was identified as a genetic determinant of plasma lipids and lipoprotein concentrations in Caucasian and North African populations [12, 13]. We aimed to analyze the association of the APOE gene polymorphisms with MetS risk and its components, by performing case-control studies for MetS, diabetes, hypertension, and obesity in a general population sample from the city of Oran in Algeria, and to highlight the potential influence of these polymorphisms on individual susceptibility to MetS. To the best of our knowledge, the association between APOE gene and MetS and related disorders had never been studied in Algeria.
The work has been done according to Helsinki Declaration, and the study’s objectives and procedures were approved by the independent ethics committee at the Algerian National Agency for the Development of Health Research (ANDRS) (since renamed as the Thematic Agency of Research in Health Sciences, ATRSS). All participants provided written informed consent prior to enrolment.
Participants were recruited during the ISOR (InSulino-résistance à ORan) study, a population-based, cross-sectional study of a representative sample of 787 individuals (378 men and 409 women, mean age 44.1 ± 10.1 years) recruited in the city of Oran, Algeria, from 2007 to 2009 .
Data were collected using a preconceived questionnaire on socioeconomic information, physical activity (The level of physical activity was defined in quartiles as “none,” “low,” “medium,” and “high” after summing exercise scores for sporting activities, walking, housework, and physical activity at work), tobacco use and alcohol intake, past medical history and family history, current medications, as well as anthropomorphic characteristics including height, weight, waist circumference, hip circumference, and blood pressure. Height and weight were measured while the subject was barefoot and lightly dressed. The BMI was calculated according to the Quetelet equation . Systolic and diastolic blood pressure values (systolic blood pressure (SBP) and diastolic blood pressure (DBP), respectively) were measured on the right arm with the subject in the sitting position, using a standard mercury sphygmomanometer. Measurements were made before and after completion of the questionnaire, with an interval of at least 10 min. The mean value of the blood pressure readings was considered for analysis. Regarding tobacco use, participants were categorized as either smokers (i.e., individuals reporting at least one cigarette per day) or non-smokers. After a 12-h overnight fast, blood was collected aseptically via venipuncture in an EDTA tube for DNA extraction and subsequent molecular analysis, and in a heparin tube for biochemistry tests .
Metabolic syndrome diagnosis criteria
In this study, we have adopted the definition of metabolic syndrome according to the criteria of the “National Cholesterol Education Program - Adult Treatment Panel III” (NCEP ATPIII) ; the metabolic syndrome is diagnosed when a subject has three or more of the following risk factors:
- Abdominal obesity: waist circumference > 102/88 cm (men/women);
- Triglyceride level ≥ 150 mg/dL (1.69 mmol/L), fibrate treatment excluded;
- HDL cholesterol < 40/50 mg/dL (1.04/1.29 mmol/L) (men/women);
- Blood pressure ≥ 135/85 mmHg or treatment for hypertension;
- Fasting glucose ≥ 110 mg/dL (6.1 mmol/L), or treatment for diabetes.
Type 2 diabetes diagnosis criteria
The definition adopted in this study is that of the American Diabetes Association (ADA) 
Fasting plasma glucose ≥ 7.0 mmol/L twice after 8 h of fasting
Occasional blood glucose ≥ 11.1 mmol/L in the presence of symptoms of hyperglycemia (polyuria, polydipsia, unexplained weight loss)
Diabetics declared under treatment including oral antidiabetic drugs and/or insulin
High blood pressure diagnosis criteria
Hypertension (HBP) has been defined according to the WHO criteria : mean systolic blood pressure [SBP] greater than 140 mmHg and/or mean diastolic blood pressure [DBP] greater than 90 mmHg, and/or self-reported current treatment for hypertension with antihypertensive drugs.
Obesity diagnosis criteria
The body mass index (BMI) is calculated according to the Quetelet equation. A subject is considered obese if he has a BMI greater than or equal to 30 kg/m2 .
Biochemistry and molecular testing
A multichannel analyzer and dedicated kits (Humastar®, HUMAN Diagnostics, Wiesbaden, Germany) were used for the colorimetric, enzymatic measurement of cholesterol (kit: monotest cholesterol with cholesterol esterase, cholesterol oxidase and peroxidase), triglycerides (kit: peridochrom triglyceride with glycerol phosphate oxidase and peroxidase), and glucose (kit: glucose, glucose oxidase, and peroxidase). Plasma LDL cholesterol levels were calculated according to the Friedewald equation. High-density lipoprotein cholesterol levels were measured after sodium phosphotungstate/magnesium chloride precipitation of chylomicrons and VLDL and LDL cholesterol and then centrifugation. Plasma insulin levels were measured using a microparticle enzyme immune assay running on an AxSYM analyzer (Abbott Laboratories, Abbott Park, Illinois, USA).
Genomic DNA was extracted from white blood cells by using the Stratagene® kit (Agilent Technologies, Les Ulis, France), according to the manufacturer’s protocol. The APOE SNPs (rs429358, rs7412, rs439401, and rs4420638) were genotyped using KASPar technology (KBioscience, Hoddesdon, UK) with the following probes:
rs4420638: [TGCTACAC TTTTCCT[A/G]GTGTGGTCTACCCGA].
The genotyping success rates ranged from 93 to 96% .
Statistical analysis was performed with SAS 9.1 software (SAS Institute Inc., Cary, NC, USA). The Hardy-Weinberg equilibrium was tested using a χ2 test with one degree of freedom (d.f.). Some of the biochemical traits (fasting glucose levels, triglycerides, and insulin levels) were not normally distributed; we therefore log-transformed these parameters to obtain normal data distributions. Intergroup comparisons of means were performed with a general linear model, and multivariate logistic regression analyses were used to calculate the odds ratios for MetS, type 2 diabetes (T2D), high blood pressure (HBP), and obesity (Obes). The confounding variables were age, gender, smoking status, and physical activity. After Bonferroni correction, only associations with an uncorrected p value below 0.017 were considered to be statistically significant (i.e., 0.05 divided by the number of polymorphisms considered).
Characteristics of study subjects
The main anthropometric, biochemical, and clinical characteristics have been measured; the baseline characteristics of the study population are described in Table 1.
Genotype and allele distributions
The allele and genotype distributions of the APOE polymorphisms were described in Table 2. There was no evidence of significant deviation from the Hardy-Weinberg equilibrium in any distributions.
Prevalence of the metabolic syndrome and the main cardiometabolic risk factors
These data concerning the Oran population are presented in Table 3.
Diabetes mellitus (T2D) was diagnosed in 80 participants (10.6%). The distribution of prevalence by sex shows no significant difference (p = 0.39); it was 11.6% for men and 9.7% for women.
The prevalence of obesity in the general population was 21.2%. It affects more women (32.5%) than men (9%), with a significant difference in the prevalence distribution between men and women (p < 0.0001).
The prevalence of the MetS in the Oran population is 20%; the distribution of this pathology is also significantly different between the two sexes (p < 0.0001). Indeed, it affects more women (25.9%) than men (13.7%).
Hypertension affects 23.1% of the study population. HBP is present in 21.2% of men and 19.6% of women; the prevalence distribution in men and women shows no significant difference (p = 0.57).
APOE epsilon polymorphism and cardiometabolic risk
No significant association was reported between genotypes of APOE epsilon polymorphism and the studied cardiovascular risk factors (T2D, obesity, HBP, and MetS status); the p values ranged from 0.04 to 0.92 (Table 4).
APOE rs439401 polymorphism and cardiometabolic risk
In the ISOR study, rs439401 showed a significant association with hypertension (HBP). The T allele increase the risk of hypertension with an odds ratio (OR) of 1.46 (95% CI [1.12–1.9], p = 0.006). No associations with T2D, obesity, and MetS were detected in the ISOR study (Table 5).
APOE rs4420638 polymorphism and cardiometabolic risk
Logistic regression analysis showed that the rs4420638 polymorphism was significantly associated with obesity in the general population. The G allele provides protection against obesity; indeed, the G allele decreases the risk of obesity, and the resulting OR is 0.48 (95% CI [0.29–0.81], p = 0.005) (Table 6). No effects of rs4420638 polymorphism on T2D, MetS, and HBP were detected in the ISOR study.
The associations described for rs439401 and rs4420638 remained significant even after adjusting for the APOE epsilon polymorphism.
To our knowledge, this is the first study that evaluates the association of APOE gene polymorphisms (epsilon, rs439401, and rs4420638), with the risk of MetS and the main cardiometabolic risk factors, within the Algerian population.
We found no association between the three polymorphisms of the APOE gene and the metabolic syndrome in the Algerian population. However, some components of the metabolic syndrome considered as cardiometabolic risk factors were significantly associated with APOE gene polymorphisms.
The logistic regression results showed that the ε2 allele increases the risk of obesity by 88% in the ISOR study. Similar results were observed in a study among the population of Croatia’s Roma minority .
It is possible that gene-nutrition interactions are responsible for the observed association between the ε2 allele and obesity. Indeed, changes in eating habits during the last decade would be responsible for increasing the prevalence of obesity, interacting with the ε2 allele [19, 20].
The polymorphisms rs439401 and rs4420638 have been associated in some of GWAS-type studies with changes in plasma lipid concentrations. The rs439401 is reportedly associated with variations in plasma lipid concentrations in a meta-analysis of genome-wide association studies (GWAS), in 16 European cohorts , whereas the rs4420638 showed similar associations in Scandinavian, Europeans ancestry, and Chinese populations [22,23,24,25], but few studies have investigated the impact of these polymorphisms on metabolic and cardiovascular traits.
Our results on the Oran population report, for the first time, that the T allele of the rs439401 polymorphism increases the risk of arterial hypertension (OR 1.46, 95% CI [1.12–1.90], p = 0.006). No similar results were reported. In the literature, the T allele of rs439401 is significantly associated with changes in BMI, insulin concentration, waist circumference, and triglyceride concentration. The TT genotype is positively associated with an increase in the values of these parameters only in psychologically stressed individuals . Our results are perhaps indicative of a state of stress of the population, resulting from the changes made in the Algerian population during the last two decades, particularly with the security crisis in the country. These hypotheses require investigations on a larger sample and, in which, the stress level must be measured accurately.
The G allele of rs4420638 seems to confer a protective effect against obesity (OR 0.48, 95% CI [0.29–0.79], p = 0.004).
No study was interested in measuring the association between rs4420638 polymorphism and obesity previously. No association was reported for the rs4420638 with MetS, T2D, and HBP; similar results were observed in a Tunisian population .
The fact that rs4420638 has low linkage disequilibrium with the epsilon polymorphism in our population gives it an advantage over European populations, where these two polymorphisms are in strong linkage disequilibrium. Thus, the study of the impact of rs4420638 would be independent of the effect of epsilon polymorphism, which makes our population very interesting from a genetic point of view for association analysis involving rs4420638 polymorphism.
Although APOE variants were not associated with the risk of MetS, the APOE polymorphism alleles were associated with some of the metabolic parameters in Algerian subjects. The relation of APOE rs439401 alleles with HBP seems indicative of a state of stress of the population. These hypotheses require in the future investigations on a larger sample and, in which, the stress level must be measured accurately.
The interaction of gene nutrition must be investigated in the future; the Algerian population shows many changes in eating habits during the last decade, which could be responsible for the increasing prevalence of obesity in our population and which can influence the effect of APOE polymorphism on the studied parameters.
Availability of data and materials
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Agence Nationale De Recherche en Santé
Agence Thématique de Recherche en Science de la Santé
Body mass index
Degree of freedom
Diastolic blood pressure
InSulino-résistance à ORan
Systolic blood pressure
Single nucleotide polymorphism
Type 2 diabetes
Reaven GM (1988) Banting lecture 1988. Role of insulin resistance in human disease. Diabetes. 37(12):1595–1607
Grundy SM, Brewer HB Jr, Cleeman JI, Smith SC Jr, Lenfant C (2004) Definition of metabolic syndrome: Report of the National Heart, Lung, and Blood Institute/American Heart Association conference on scientific issues related to definition. Circulation. 109(3):433–438
Gami AS, Witt BJ, Howard DE, Erwin PJ, Gami LA, Somers VK et al (2007) Metabolic syndrome and risk of incident cardiovascular events and death: a systematic review and meta-analysis of longitudinal studies. Journal of the American College of Cardiology. 49(4):403–414
Kahn R, Buse J, Ferrannini E, Stern M (2005) The metabolic syndrome: time for a critical appraisal: joint statement from the American Diabetes Association and the European Association for the Study of Diabetes. Diabetes care. 28(9):2289–2304
Meigs JB, Rutter MK, Sullivan LM, Fox CS, D'Agostino RB Sr, Wilson PW (2007) Impact of insulin resistance on risk of type 2 diabetes and cardiovascular disease in people with metabolic syndrome. Diabetes care. 30(5):1219–1225
Wilson PW, D'Agostino RB, Parise H, Sullivan L, Meigs JB (2005) Metabolic syndrome as a precursor of cardiovascular disease and type 2 diabetes mellitus. Circulation. 112(20):3066–3072
Hillier TA, Rousseau A, Lange C, Lepinay P, Cailleau M, Novak M et al (2006) Practical way to assess metabolic syndrome using a continuous score obtained from principal components analysis. Diabetologia. 49(7):1528–1535
Ministère de la Santé et de la réforme hospitalière. Transition épidémiologique et système de santé. Projet TAHINA. report 2007 Mai 2007. Report No.
Balkau B, Valensi P, Eschwege E, Slama G (2007) A review of the metabolic syndrome. Diabetes & metabolism. 33(6):405–413
Houti L, Hamani-Medjaoui I, Lardjam-Hetraf SA, Ouhaibi-Djellouli H, Chougrani S, Goumidi L et al (2016) Prevalence of metabolic syndrome and its related risk factors in the city of Oran, Algeria: the ISOR Study. Ethnicity & disease. 26(1):99–106
Chuang LM (2008) Human genetics of the metabolic syndrome. Asia Pacific journal of clinical nutrition. 17(Suppl 1):43–46
Wilson PW, Myers RH, Larson MG, Ordovas JM, Wolf PA, Schaefer EJ (1994) Apolipoprotein E alleles, dyslipidemia, and coronary heart disease. The Framingham Offspring Study. Jama. 272(21):1666–1671
Boulenouar H, Benchekor SM, Meroufel DN, Hetraf SAL, Djellouli HO, Hermant X et al (2013) Impact of APOE gene polymorphisms on the lipid profile in an Algerian population. Lipids Health Dis. 12
Keys A, Fidanza F, Karvonen MJ, Kimura N, Taylor HL (1972) Indices of relative weight and obesity. J Chron Dis 25:329–343
Alberti KG, Zimmet P, Shaw J (2005) The metabolic syndrome-a new worldwide definition. Lancet. 366(9491):1059–1062
Gavin JR, 3rd. New classification and diagnostic criteria for diabetes mellitus. Clinical cornerstone. 1998;1(3):1-12.
Chalmers J, MacMahon S, Mancia G, Whitworth J, Beilin L, Hansson L et al (1999) 1999 World Health Organization-International Society of Hypertension Guidelines for the management of hypertension. Guidelines sub-committee of the World Health Organization. Clin Exp Hypertens 21(5-6):1009–1060
Zeljko HM, Skaric-Juric T, Narancic NS, Tomas Z, Baresic A, Salihovic MP et al (2011) E2 allele of the apolipoprotein E gene polymorphism is predictive for obesity status in Roma minority population of Croatia. Lipids Health Dis. 10:9
Boer JM, Ehnholm C, Menzel HJ, Havekes LM, Rosseneu M, O'Reilly DS et al (1997) Interactions between lifestyle-related factors and the APOE polymorphism on plasma lipids and apolipoproteins. The EARS Study. European Atherosclerosis Research Study. Arteriosclerosis, thrombosis, and vascular biology. 17(9):1675–1681
Talmud PJ (2007) Gene-environment interaction and its impact on coronary heart disease risk. Nutrition, metabolism, and cardiovascular diseases: NMCD 17(2):148–152
Aulchenko YS, Ripatti S, Lindqvist I, Boomsma D, Heid IM, Pramstaller PP, Penninx BW, Janssens AC, Wilson JF, Spector T et al (2009) Loci influencing lipid levels and coronary heart disease risk in 16 European population cohorts. Nat Genet. 41:47–55
Teslovich TM, Musunuru K, Smith AV, Edmondson AC, Stylianou IM, Koseki M et al (2010) Biological, clinical and population relevance of 95 loci for blood lipids. Nature. 466:707–713
Kathiresan S, Melander O, Guiducci C, Surti A, Burtt NP, Rieder MJ et al (2008) Six new loci associated with blood low-density lipoprotein cholesterol, high-density lipoprotein cholesterol or triglycerides in humans. Nat Genet. 40:189–197
Willer CJ, Speliotes EK, Loos RJ, Li S, Lindgren CM, Heid IM, Berndt SI et al (2009) Six new loci associated with body mass index highlight a neuronal influence on body weight regulation. Nat Genet. 41:25–34
Liu Y, Zhou D, Zhang Z, Song Y, Zhang D, Zhao T et al (2011) Effects of genetic variants on lipid parameters and dyslipidemia in a Chinese population. J Lipid Res 52:354–360
Kring SI, Brummett BH, Barefoot J, Garrett ME, Ashley-Koch AE, Boyle SH et al (2010) Impact of psychological stress on the associations between apolipoprotein E variants and metabolic traits: findings in an American sample of caregivers and controls. Psychosomatic medicine. 72(5):427–433
Elouej S, Rejeb I, Attaoua R, Nagara M, Sallem OK, Kamoun I et al (2016) Gender-specific associations of genetic variants with metabolic syndrome components in the Tunisian population. Endocrine research. 41(4):300–309
We are grateful to Dr Louisa Goumidi, Dr Aline Meirhaeghe, and Pr Philippe Amouyel for the technical support in DNA extraction, genotyping, and statistical analysis, which were performed in their lab (INSERM, U744; Institut Pasteur de Lille, Université Lille Nord de France, Lille, France)
The work in Algeria (patient recruitment, biochemical analysis, and DNA extraction) was partly funded by the Algerian National Agency for the Development of Health Research (ANDRS) and a grant from the Projets Nationaux de Recherche (PNR) program run by the Algerian Direction Générale de la Recherche Scientifique et du Développement Technologique/ Ministère de l’Enseignement Supérieur et de la Recherche Scientifique (DGRSDT/MESRS).
The ISOR project was funded through a collaboration agreement between the Direction de la Post-Graduation et de la Recherche-Formation (DPGRF) (Algeria) and the Institut National de la Santé et de la Recherche Médicale (INSERM) (France), which financed the scientific stays of Algerian and French researchers from and to France.
The work in France (genotyping) was also partly funded by INSERM.
Ethics approval and consent to participate
The work has been done according to Helsinki Declaration and the study’s objectives and procedures were approved by the independent ethics committee at the Algerian National Agency for the Development of Health Research (ANDRS) (reference n°02/07/01/01/076), (since renamed as the Thematic Agency of Research in Health Sciences, ATRSS). All participants provided written informed consent prior to enrolment.
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The authors declare that they have no competing interests.
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Boulenouar, H., Mediene Benchekor, S., Ouhaibi Djellouli, H. et al. Association study of APOE gene polymorphisms with diabetes and the main cardiometabolic risk factors, in the Algerian population. Egypt J Med Hum Genet 20, 5 (2019) doi:10.1186/s43042-019-0013-6
- High blood pressure
- Metabolic syndrome
- Algerian population